Rabbi Moshe ben Machir, also known as Moshe ben Yehudah haMachiri, was one of the sages and kabbalists of Safed in the 16th century. He was a close friend of Rabbi Shmuel de Uceda, author of Midrash Shmuel on Pirkei Avot.

He headed a yeshiva in Ein Zeitim (Ein Zeitoun), near Safed, where the Tanna Rabbi Yehudah bar Ilai and other sages are buried. The yeshiva was apparently located there from 5342 (1582). Fundraising letters it sent abroad between 1598 and 1601, together with a manuscript of its rules, show the typical structure of a Sephardic yeshiva of the period: a circle of scholars led by a master known as the "Rishon" or the "Chacham".

Like the other circles of Safed, it was marked by the intense spirituality and messianic expectation of the time, with a focus on repentance and ascetic practice. Unlike the circles of Rabbi Shlomo Alkabetz, the Arizal, Rabbi Chaim Vital and Rabbi Elazar Azikri, which centered on the study of mystical secrets, it followed the traditional learning of the Sephardic yeshivot. Its ten rules set up shifts so that Torah was studied day and night, covering Mishnah, Zohar, Gemara, Scripture and the works of the Rif and the Rambam, and established a weekly visit to the grave of Rabbi Yehudah bar Ilai to pray "for all Israel". In 5361 (1601), after an attack by raiders, the yeshiva moved from Ein Zeitim to Safed.

He is best known for Seder HaYom, a work of halacha and kabbalah which describes, in the words of its introduction, the order a person should follow by day and by night, on Shabbat and festivals, throughout the year. His son-in-law, Rabbi Shlomo Mor David, had it printed for the first time in Venice in 5359 (1599) while raising funds there for his father-in-law's yeshiva, and it was reprinted in Venice in 1605. Cited by many halachic authorities, it is the source of numerous customs, including the Modeh Ani prayer, which appears for the first time in this book. It also contains a commentary on the mishnah "Ben chamesh shanim lamikra" and a commentary on Ecclesiastes.