Rabbi David Shlomo Eibeschitz was born around 5515 (1755) in Ozeran (Jezierzany, in Galicia) to Rabbi Yerachmiel Eibeschitz. Recognized as a prodigy, he was chosen as a son-in-law, on the advice of Rabbi Meir Margolis, rabbi of Ostroh and Lemberg, by the latter's nephew Rabbi Yechiel Michel Margolis, rabbi of Leshkovitz. After his marriage he lived at his father-in-law's table and prepared for print the writings of his wife's grandfather, Rabbi Moshe Avraham Abush Margolis, later published in 5581 (1821) as Tziluta DeAvraham.

Drawn to Chassidut, he is counted among the disciples of Rabbi Yechiel Michel of Zlotchov and frequented Rabbi Zev Wolf of Tcherny-Ostrog; his principal teacher was Rabbi Meshulam Feivush Heller of Zbarazh. In his early twenties he was invited to Nadvorna as rosh yeshiva, where he raised many students while leading a life of asceticism and fasting.

He went on to serve as rabbi of Khorostkov and then, for at least ten years, of Budzanov, where he fought the influence of the Haskalah with fiery sermons. He finally settled in Soroki in Bessarabia, apparently its first rabbi, where he headed the rabbinical court and founded the first yeshiva in Bessarabia, becoming known as "the Rav of Soroki".

In 5568 (1808) he emigrated to the Land of Israel and settled in Safed, where he set aside all rabbinic titles and lived in poverty as a simple children's teacher, his neighbors unaware of his stature. Works: Levushei Serad on the Shulchan Aruch, the only book printed in his lifetime, whose glosses appear in most editions of the code; Arvei Nachal, a widely reprinted collection of Chassidic discourses on the Torah; and the responsa Neot Desheh.

He passed away in Safed on 22 Cheshvan 5574 (November 15, 1813), aged about sixty, and was buried in a burial cave in the old cemetery of Safed beside Rabbi Aryeh Leib of Volochisk; Rabbi Avraham Dov of Avritch was laid to rest there years later.